| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 81 | Clostridium tertium | AGGCTGATTGGAGTAGTGGC | TGTGGAATTCCCTTTGGGCA | 59.46 | 59.81 | 235 | 127 |
100.00%
|
100.00%
|
| Primer 82 | Clostridium tertium | TGGTACTCCTGCACACACA | CCCAATCTCCCCACCAAGT | 58.48 | 58.91 | 197 | 126 |
100.00%
|
100.00%
|
| Primer 83 | Clostridium tertium | GCGCAGGTAAAGGAAGCA | TGCAACAGCCTCTGCACA | 58.02 | 59.81 | 188 | 126 |
100.00%
|
100.00%
|
| Primer 84 | Clostridium tertium | ACGAAAGTGCTCGAGGAACA | AGCATTTGCAAAGCCCTGT | 59.61 | 58.56 | 216 | 126 |
100.00%
|
100.00%
|
| Primer 85 | Clostridium tertium | GTATGGGGTGGAAAAGGTGAG | ACCAATCTCAACACCCGTAAC | 58.28 | 58.23 | 174 | 126 |
100.00%
|
100.00%
|
| Primer 86 | Clostridium tertium | GGGGTGTTTGGATTGGCAG | TGCAGCAGGTACAACAACC | 59.03 | 58.29 | 231 | 125 |
100.00%
|
100.00%
|
| Primer 87 | Clostridium tertium | GGGGTGTTTGGATTGGCAG | TTGCAGCAGGTACAACAACC | 59.03 | 58.97 | 232 | 125 |
100.00%
|
100.00%
|
| Primer 88 | Clostridium tertium | TGCAACAGCATCCATAGCCTT | TGCCGTTATAGATAGCAGGCT | 60.34 | 58.75 | 244 | 125 |
100.00%
|
100.00%
|
| Primer 89 | Clostridium tertium | GCAACAGCATCCATAGCCTT | TGCCGTTATAGATAGCAGGCT | 58.60 | 58.75 | 243 | 125 |
100.00%
|
100.00%
|
| Primer 90 | Clostridium tertium | GGAAGCTCTGACACAACAGC | AGTGCTAGTACCCCTGCAGA | 59.13 | 59.96 | 233 | 125 |
100.00%
|
100.00%
|